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KMID : 0603820170230010025
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Journal of Experimental & Biomedical Science
2017 Volume.23 No. 1 p.25 ~ p.29
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Effect of Antioxidants on Arsenite Exposed Porcine Embryonic Development
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Kim Han-Su
Lee Yu-Sub
Lee Sang-Hee
Cheong Hee-Tae
Park Choon-Keun
Lee Seung-Hyung
Yang Boo-Keun
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Abstract
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This study was to investigate the role of antioxidants on development in arsenite exposed porcine embryos. Oocytes were collected from porcine ovary, and then matured for 44 h. Maturated oocytes were incubated with sperm for 6 h, and fertilized oocytes with sperm (embryos) cultured for 48 h. After, embryos were culture with arsenite and/or antioxidants (melatonin, silymarin, curcumin and vitamin) for 120 h. Formation of pre-morulae, morulae and blastosysts rate was measured using microscope. In results, 10, 100 and 100 nM arsenite significantly decreased morulae and blastocysts formation compared to control in pigs (P<0.05). 10 μM silymarin and 100 μM vitamin E increased blastocyst formation compared to 10 nM arsenite exposed embryos, but there were no significantly among the treatment, and 1 nM melatonin and 5 μM curcumin did not influence blastocysts formation in 10 nM arsenite exposed embryos. In summary, arsenite decreased embryo development, 10 μM silymarin, 100 μM vitamin E, 1 nM melatonin and 5 μM curcumin had no positive effect to blastocyst formation in arsenite exposed porcine embryos. Therefore, we suggest that little arsenite may have negative effect to embryo development, and silymarin, vitamin E, melatonin and curcumin could not rescue embryo development from damage by arsenite in pigs.
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KEYWORD
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Embryo development , Arsenite , Antioxidants , Pigs
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